Enzymatic nitrile hydrolysis catalyzed by nitrilase ZmNIT2 from maize. An unprecedented β-hydroxy functionality enhanced amide formation
C Mukherjee, D Zhu, ER Biehl, RR Parmar, L Hua
Index: Tetrahedron, , vol. 62, # 26 p. 6150 - 6154
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Citation Number: 22
Abstract
... Tetrahedron. ... Because the crystal structure of nitrilase ZmNIT2 is not known, how the intra-molecular hydrogen bonding in the tetrahedral intermediate (2 ... The cell-free extract was mixed with equal volume of PEI solution (0.25% polyethyleneimine MW 40–60K, 6% NaCl, 100 ...
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