Synthesis of β-d-fructofuranosyl-(2→ 1)-2-acetamido-2-deoxy-α-d-glucopyranoside (N-acetylsucrosamine) using β-fructofuranosidase-containing Aspergillus oryzae …

…, M Juami, M Nakamura, Y Kumaki, W Hakamata…

Index: Hirano, Takako; Wada, Toru; Iwai, Sumire; Sato, Hitoshi; Noda, Makoto; Juami, Mai; Nakamura, Masatoshi; Kumaki, Yasuko; Hakamata, Wataru; Nishio, Toshiyuki Carbohydrate Research, 2012 , vol. 353, p. 27 - 32

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Citation Number: 7

Abstract

Using soft granules consisting of Celite 535 and dried Aspergillus oryzae NBRC100959 mycelia containing β-fructofuranosidase as a whole-cell catalyst, N-acetylsucrosamine [β-d- fructofuranosyl-(2→ 1)-2-acetamido-2-deoxy-α-d-glucopyranoside] was produced from sucrose and 2-acetamido-2-deoxy-d-glucose by enzymatic transfructosylation. The isolated yield of N-acetylsucrosamine from the reaction mixture was 22.1%(from sucrose). The ...

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