Analytical chemistry

Conjugated polyelectrolyte based real-time fluorescence assay for phospholipase C

Y Liu, K Ogawa, KS Schanze

Index: Liu, Yan; Ogawa, Katsu; Schanze, Kirk S. Analytical Chemistry, 2008 , vol. 80, # 1 p. 150 - 158

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Citation Number: 80

Abstract

A fluorescence turnoff assay for phospholipase C (PLC) from Clostridium perfringens is developed based on the reversible interaction between the natural substrate, phosphatidylcholine, and a fluorescent, water-soluble conjugated polyelectrolyte (CPE). The fluorescence intensity of the CPE in water is increased substantially by the addition of the phospholipid due to the formation of a CPE-lipid complex. Incubation of the CPE-lipid ...

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