Biotechnological production of high specific activity L??35 S??cysteine and L??35 S??methionine by using a diploid yeast Saccharomyces cerevisiae

N Gajendiran, N Jayachandran, BS Rao…

Index: Gajendiran; Jayachandran; Rao; Unny; Thyagarajan Journal of Labelled Compounds and Radiopharmaceuticals, 1994 , vol. 34, # 6 p. 571 - 576

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Citation Number: 6

Abstract

Abstract High specific activity L-35 S-cysteine and L-35 S-methionine were synthesised by using a wild type diploid strain of baker's yeast—Saccharomyces cerevisiae. Yeast cells were grown in a sulphur depleted synthetic medium in which Na 2 35 SO 4 (50 mCi/ml) was supplemented as the sole sulphur source. The level of incorporation was 60% on an average. The protein hydrolysate of the cultured cells was subjected to paper and column ...