Characterization of the Immobilized β-Galactosidase C from Bacillus circulans and the Production of β (1→ 3)-linked Disaccharides

A Naundorf, M Caussette, K Ajisaka

Index: Naundorf, Andreas; Caussette, Mylene; Ajisaka, Katsumi Bioscience, Biotechnology and Biochemistry, 1998 , vol. 62, # 7 p. 1313 - 1317

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Citation Number: 21

Abstract

A recombinant β-galactosidase, which was obtained from the β-galactosidase C gene of Bacillus circulans and cleaves the non-reducing end galactosyl residue of β (1→ 3)-linkages selectively, was immobilized using CNBr-Sepharose. Although the effect of pH was not changed by the immobilization, the thermostability and stability in the presence of DMF were increased. Optimization of the transglycosylation using para-nitrophenyl β-d- ...

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