Biocatalytic Enantioselective Synthesis of N??Substituted Aspartic Acids by Aspartate Ammonia Lyase

B Weiner, GJ Poelarends, DB Janssen…

Index: Weiner, Barbara; Poelarends, Gerrit J.; Janssen, Dick B.; Feringa, Ben L. Chemistry - A European Journal, 2008 , vol. 14, # 32 p. 10094 - 10100

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Citation Number: 41

Abstract

Abstract The gene encoding aspartate ammonia lyase (aspB) from Bacillus sp. YM55-1 has been cloned and overexpressed, and the recombinant enzyme containing a C-terminal His 6 tag has been purified to homogeneity and subjected to kinetic characterization. Kinetic studies have shown that the His 6 tag does not affect AspB activity. The enzyme processes L- aspartic acid, but not D-aspartic acid, with a K m of≈ 15 m M and ak cat of≈ 40 s− 1. By ...

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