Analytical biochemistry

A chromogenic substrate for solid-phase detection of phospholipase A 2

C Eba, A Okano, H Nakano, Y Iwasaki

Index: Eba, Chisato; Okano, Aoi; Nakano, Hideo; Iwasaki, Yugo Analytical Biochemistry, 2014 , vol. 447, # 1 p. 43 - 45

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Citation Number: 2

Abstract

Abstract A method for solid-phase detection of phospholipase A 2 (PLA 2) was developed. The method uses 1-octanoyloxynaphthalene-3-sulfonic acid, which was found to be a good substrate of PLA 2. The substrate is hydrolyzed by PLA 2 into 1-naphthol-3-sulfonic acid, which is spontaneously coupled with coexisting diazonium salt to form a red-purple azo dye. Streptomyces and bovine pancreatic PLA 2 spotted on a nitrocellulose membrane could ...