A macrocyclic coumarin-containing tripeptide via CuAAC chemistry
SS van Berkel, B van der Lee, FL van Delft…
Index: Van Berkel, Sander S.; Van Der Lee, Bas; Van Delft, Floris L.; Rutjes, Floris P. J. T. Chemical Communications, 2009 , # 28 p. 4272 - 4274
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Citation Number: 3
Abstract
We envisaged a fluorophore-containing cyclic structure (ie2, Fig. 1) as a compelling strategy to (a) overcome the poor solubility of AMC in physiological media and (b) give rise to improved kinetic parameters. Constraining the linear peptide H-Gly-Pro-Arg-AMC in a bulky macrocyclic conformation is expected to hamper the entry into the thrombin active site, thus decreasing the affinity of the substrate for the binding pocket of thrombin resulting in favourable kinetic ...
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