Dynamics at the active site of N2-acetyl-N1-(4-fluorobenzyl) carbazoyl-. alpha.-chymotrypsin

JT Gerig, SJ Hammond

Index: Gerig, J. T.; Hammond, S. J. Journal of the American Chemical Society, 1984 , vol. 106, # 26 p. 8244 - 8251

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Citation Number: 16

Abstract

Abstract:~-Acety1-N1-(4-fluorobenzyl) carbazoy1-a-chymotrypin is a catalytically inactive protein which should closely resemble the acylated enzyme intermediate formed during reaction of chymotrypsin with substrates derived from phenylalanine. Fluorine and deuterium nuclear relaxation in this carbazoylated enzyme and a specifically deuterated analogue have been examined. Analysis of the spin-lattice relaxation data (observed at ...