Protection and labelling of thymidine by a fluorescent photolabile group

C Muller, P Even, ML Viriot, MC Carré

Index: Muller, Caroline; Even, Pascale; Viriot, Marie-Laure; Carre, Marie-Christiane Helvetica Chimica Acta, 2001 , vol. 84, # 12 p. 3735 - 3741

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Citation Number: 40

Abstract

Abstract A fluorescent photolabile group including coumarin and MeNPOC moieties was synthesized to protect 5′-OH terminal function of thymidine (T). Its photochemical and photophysical properties were studied, in particular the photocleavage (photodeprotection under a 365-nm irradiation) is only lowered by a factor of two by addition of the fluorophore. Fluorescence properties of the coumarin probe are not changed upon irradiation, which is ...