Chemical communications

A high-throughput screening assay for hydroxynitrile lyase activity

J Andexer, JK Guterl, M Pohl, T Eggert

Index: Andexer, Jennifer; Guterl, Jan-Karl; Pohl, Martina; Eggert, Thorsten Chemical Communications, 2006 , # 40 p. 4201 - 4203

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Citation Number: 30

Abstract

Hydroxynitrile lyases (HNLs) naturally occur in plants integrated in microbial and herbivore defense mechanisms by HCN-release due to cyanohydrin cleavage. Some HNLs are biochemically characterized in detail concerning substrate specificity and enantioselectivity. The HNLs from Prunus species, like P. amygdalus (bitter almond) 1a and P. mume (Japanese apricot) 1b as well as the HNL from Linum usitatissimum (flax) 1c show (R)-selectivity, whereas the other HNLs ...

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