Inhibitors of an AdoMet-dependent 3-amino-3-carboxypropyl transferase and their use as ligands for protein affinity chromatography

AM Reeve, CA Townsend

Index: Reeve, Anne M.; Townsend, Craig A. Tetrahedron, 1998 , vol. 54, # 52 p. 15959 - 15974

Full Text: HTML

Citation Number: 14

Abstract

The screening of potential affinity ligands and the development of a two-stage affinity purification of an S-adenosylmethionine-dependent 3-amino-3-carboxypropyl transferase involved in the biosynthesis of the β-lactam antibiotic nocardicin A is described from a cell- free extract of Nocardia uniformis subsp. tsuyamanesis. This protein is particularly sensitive to ion exchange and salt effects necessitating a pair of neutral ligands to be used in the ...