Synthesis of a caged glutamate for efficient one-and two-photon photorelease on living cells

…, JY Sul, PG Haydon, GCR Ellis-Davies

Index: Fedoryak, Olesya D.; Sul, Jai-Yoon; Haydon, Philip G.; Ellis-Davies, Graham C. R. Chemical Communications, 2005 , # 29 p. 3664 - 3666

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Citation Number: 33

Abstract

We have synthesized a biologically inert, photosensitive derivative of the major excitatory amino acid, L-glutamate (which we call MDNI-glu) that makes more efficient use of incident light than all other caged glutamates. Laser flash photolysis of MDNI-glu in acutely isolated hippocampal brain slices evoked a rapid increase in intracellular Ca2+ concentration in astrocytes.