Immobilization of lipase on Fe 3 O 4/ZnO core/shell magnetic nanoparticles and catalysis of Michael-type addition to chalcone derivatives

S Ghasemi, M Heidary, MA Faramarzi…

Index: Ghasemi, Sabrieh; Heidary, Marjan; Faramarzi, Mohammad Ali; Habibi, Zohreh Journal of Molecular Catalysis B: Enzymatic, 2014 , vol. 100, p. 121 - 128

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Citation Number: 20

Abstract

Abstract Fe 3 O 4/ZnO core/shell magnetic nanoparticles in 1: 1 and 1: 2 molar ratios were prepared and characterized by using X-ray powder diffraction, scanning electron microscopy, thermogravimetric analysis, FT-IR spectra, and flame atomic absorption spectrophotometry. Thermomyces lanuginosa lipase (TLL) was then covalently immobilized on the surface of both supports. An immobilization efficiency and yield of 94±2.5% and 86 ...