Development of an affinity ligand for purification of. alpha. 2-adrenoceptors from human platelet membranes

…, WD Matthews, JW Regan, RJ Lefkowitz…

Index: DeMarinis; Krog; Shah; Lafferty; Holden; Hieble; Matthews; Regan; Lefkowitz; Caron Journal of Medicinal Chemistry, 1984 , vol. 27, # 7 p. 918 - 921

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Citation Number: 22

Abstract

Discussion In the technique of affinity chromatography, a ligand with high affinity for the receptor of interest is immobilized via a stable covalent linkage onto an insoluble support matrix, such as agarose. This ligand is usually attached via a linear hydrophilic spacer arm such that the ligand is free to float in the void of the resin where it is accessible to the receptor of interest. A solubilized receptor preparation is placed on the column and ...