Bicyclomycin fluorescent probes: synthesis and biochemical, biophysical, and biological properties

AP Brogan, WR Widger, H Kohn

Index: Brogan, Andrew P.; Widger, William R.; Kohn, Harold Journal of Organic Chemistry, 2003 , vol. 68, # 14 p. 5575 - 5587

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Citation Number: 16

Abstract

Bicyclomycin (1) is a commercially available antibiotic whose primary site of action in Escherichia coli is the transcription termination factor rho. Key aspects of the 1⊙ rho interaction K d, stoichiometry for 1⊙ rho binding, and whether 1 and ATP binding induce conformational changes in rho remain unknown. In this study, the design, synthesis, and characterization of a series of bicyclomycin fluorescent probes (BFP) constructed to sense ...