Bioorganic & medicinal chemistry letters

Development of an affinity-driven cross-linker: isolation of a vitamin D receptor associated factor

N Swamy, JK Addo, R Ray

Index: Swamy, Narasimha; Addo, James K.; Ray, Rahul Bioorganic and Medicinal Chemistry Letters, 2000 , vol. 10, # 4 p. 361 - 364

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Citation Number: 10

Abstract

A vitamin D analogue containing an affinity and a photoaffinity probe (affinity-driven cross- linker, Double Label) was synthesized. An unknown factor, associated with vitamin D receptor (VDR), was isolated from rat liver nuclear extract using a GST-VDR-ligand-binding domain fusion protein (GST-VDR-LBD), affinity labeled with Double Label, and protein– protein cross-linking by photolysis.