3??Mercapto??2, 6??Pyridinedicarboxylic Acid: A Small Lanthanide??Binding Tag for Protein Studies by NMR Spectroscopy

…, XC Su, H Liang, S Simonsen, T Huber…

Index: Man, Bradley; Su, Xun-Cheng; Liang, Haobo; Simonsen, Shane; Huber, Thomas; Messerle, Barbara A.; Otting, Gottfried Chemistry - A European Journal, 2010 , vol. 16, # 12 p. 3827 - 3832

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Citation Number: 37

Abstract

Abstract Paramagnetic effects from lanthanide ions present powerful tools for protein studies by nuclear magnetic resonance (NMR) spectroscopy provided that the lanthanide can be site-specifically and rigidly attached to the protein. A new, particularly small and rigid lanthanide-binding tag, 3-mercapto-2, 6-pyridinedicarboxylic acid (3MDPA), was synthesized and attached to two different proteins via a disulfide bond. The complexes of ...