Chemical Communications

Biocatalytic synthesis of uridine 5′-diphosphate N-acetylglucosamine by multiple enzymes co-immobilized on agarose beads

J Shao, J Zhang, J Nahálka, PG Wang

Index: Shao, Jun; Zhang, Jianbo; Nahalka, Jozef; Wang, Peng George Chemical Communications, 2002 ,  # 21  p. 2586 - 2587

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Citation Number: 12

Abstract

Recombinant N-acetylglucosamine kinase, pyruvate kinase, N-acetylglucosamine phosphate mutase, uridine 5′-diphosphate N-acetylglucosamine pyrophosphorylase, and inorganic pyrophosphatase were overexpressed in E. coli and co-immobilized on agarose beads for the practical synthesis of uridine 5′-diphosphate N-acetylglucosamine.