Solid-phase synthesis of positively charged deoxynucleic guanidine (DNG) oligonucleotide mixed sequences

PM Reddy, TC Bruice

Index: Reddy, Putta Mallikarjuna; Bruice, Thomas C. Bioorganic and Medicinal Chemistry Letters, 2003 ,  vol. 13,  # 7  p. 1281 - 1285

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Citation Number: 25

Abstract

Positively charged DNG oligonucleotide mixed sequences containing A/T bases were prepared by solid-phase synthesis. Synthesis proceeds in 3′→ 5′ direction and involves coupling of 3′-Fmoc protected thiourea in the presence of HgCl2/TEA with the corresponding 5′-amine of the growing oligo chain. DNG binding characteristics with complementary DNA and with itself have been evaluated.