Cross-linked crystals of Candida rugosa lipase: highly efficient catalysts for the resolution of chiral esters

…, C Govardhan, N Khalaf, AG Martinez…

Index: Lalonde, Jim J.; Govardhan, Chandrika; Khalaf, Nazer; Martinez, Aldo G.; Visuri, Kalevi; Margolin, Alexey L. Journal of the American Chemical Society, 1995 ,  vol. 117,  # 26  p. 6845 - 6852

Full Text: HTML

Citation Number: 176

Abstract

Abstract: To date, most enzyme-based organic syntheses have employed enzymes in the form of a crude protein extract. The instability and expense of highly purified proteins has all but obviated their use as catalysts for enantioselective hydrolyses. Herein, we describe the use of the major hydrolase from commercial Candida rugosa lipase (CRL) in the form of a cross-linked enzyme crystal (CLEC) for the enantioselective hydrolysis of chiral racemic ...