Synthesis and microbiological activities of some monohalogenated analogs of tyrosine
…, JF Grimes, KL Hulme, LQ Robinson…
Index: McCord; Smith; Winters; Grimes; Hulme; Robinson; Gage; Davis Journal of Medicinal Chemistry, 1975 , vol. 18, # 1 p. 26 - 29
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Citation Number: 18
Abstract
Enzyme Preparation. The preparation of guinea pig myocw dial adenylate cyclase fractions has been previously described. 14 Briefly. the enzyme preparation is a once-washed particulate fraction derived from a 20% homogenate of ventricular tissue in MgClz (1 mM)- glycylglycine (2 mM) buffer (pH 7.5), by centrifugation at l00Og for 15 min at 4". Aliquots (0.5 ml) of the washed and resuspended pellets from the centrifugation were sealed in ...
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