Regioisomeric characterization of triacylglycerols using silver-ion HPLC/MS and randomization synthesis of standards

M Lísa, H Velínská, M Holčapek

Index: Lisa, Miroslav; Velinska, Hana; Holcapek, Michal Analytical Chemistry, 2009 , vol. 81, # 10 p. 3903 - 3910

Full Text: HTML

Citation Number: 62

Abstract

Silver-ion normal-phase high-performance liquid chromatography (HPLC) provides a superior separation selectivity for lipids differing in the number and position of double bonds in fatty acid chains including the resolution of triacylglycerol (TG) regioisomers under optimized conditions. Our silver-ion HPLC method is based on the coupling of three columns in the total length of 75 cm and a new mobile phase gradient consisting of hexane− ...