Purification and properties of β-cyano-l-alanine synthase from Vicia angustifolia
F Ikegami, K Takayama, T Kurihara, S Horiuchi…
Index: Ikegami, Fumio; Takayama, Kyoko; Tajima, Chiho; Murakoshi, Isamu Phytochemistry (Elsevier), 1988 , vol. 27, # 7 p. 2011 - 2016
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Abstract
Abstract β-Cyano-l-alanine synthase was purified ca 1000-fold to homogeneity from the immature seeds of Vicia angustifolia. The purified enzyme has an apparent M r of 54 000 consisting of two identical subunits. The subunits contain one molecule of pyridoxal 5′- phosphate each. The K m value is 3.6 mM for l-cysteine and 0.5 mM for cyanide. β-Cyano-l- alanine synthase from V. angustifolia also catalyses the formation of some S-substituted l- ...
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