A high-throughput, low-volume enzyme assay on solid support

P Babiak, JL Reymond

Index: Babiak, Peter; Reymond, Jean-Louis Analytical Chemistry, 2005 , vol. 77, # 2 p. 373 - 377

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Citation Number: 55

Abstract

A high-throughput enzyme assay is described that uses 1 μL or less of enzyme solution for each test. Enzyme solutions are deposited by robotic handling in a throughput of over 1000 tests/h on the surface of silica gel plates that have been preimpregnated with fluorogenic substrates. The reaction is quantitated by fluorescence. The method is compatible with water- insoluble substrates (lipases), water-soluble substrates (glycosidases), whole-protein ...