Photoreactivation of irreversibly inhibited serine proteinases

AD Turner, SV Pizzo, G Rozakis…

Index: Turner,A.D.; Pizzo,S.V.; Rozakis,G. Journal of the American Chemical Society, 1988 , vol. 110, p. 244

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Citation Number: 67

Abstract

Abstract: The p-amidinophenyl ester of@ hydroxy-a-methylcinnamic acid has been synthesized and characterized. This compound irreversibly inhibits thrombin, Factor Xa, and trypsin. Inhibition by this derivative is, however, reversible (80-100%) upon irradiation of the enzyme in the presence of excess compound. Photolysis of the inactive 1: l acylenzyme complexes of all three enzymes (purified to homogeneity) also regenerated full enzymatic ...