Glutamyl adenylate analogues are inhibitors of glutamyl-tRNA synthetase

M Desjardins, S Garneau, J Desgagnés, L Lacoste…

Index: Desjardins, Michel; Garneau, Sylvie; Desgagnes, Julie; Lacoste, Lucille; Yang, Fu; Lapointe, Jacques; Chenevert, Robert Bioorganic Chemistry, 1998 , vol. 26, # 1 p. 1 - 13

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Citation Number: 21

Abstract

Glutamol adenylate10was a competitive inhibitor (Ki= 3 μM) of glutamyl-tRNA synthetase fromEscherichia coli. TheN6-benzoyl adenine derivative9was also an inhibitor (Ki∼ 60 μM). Replacement of adenine by other bases (purine, cytosine, dihydrocytosine, uridine) resulted in a more than 1000-fold loss in activity, indicating the important contribution of the adenine ring to the enzyme binding.