Cyclen based lanthanide ion ribonuclease mimics: the effect of pyridine cofactors upon phosphodiester HPNP hydrolysis
…, T McCabe, S Mulready, JE O'Brien, CS Stevenson…
Index: Gunnlaugsson, Thorfinnur; Davies, R. Jeremy H.; Kruger, Paul E.; Jensen, Paul; McCabe, Thomas; Mulready, Sinead; O'Brien, John E.; Stevenson, Clarke S.; Fanning, Ann-Marie Tetrahedron Letters, 2005 , vol. 46, # 21 p. 3761 - 3766
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Citation Number: 22
Abstract
The cyclen based pyridine complexes (Ln= La (III) and Eu (III)) were synthesised as metallo- ribonuclease mimics and their ability to hydrolytically cleave the phosphodiester of HPNP at 37° C was investigated using UV–vis spectroscopy, whereas the binding of the substrate was evaluated using 31P NMR and Eu (III)-luminescent measurements. In contrast gave rise to fast pH dependent hydrolysis of HPNP, with maximum efficiency at ca. pH8. 2, and with ...