Purification and cloning of a γ-glutamyl transpeptidase from onion (Allium cepa)

ML Shaw, MD Pither-Joyce, JA McCallum

Index: Shaw, Martin L.; Pither-Joyce, Meeghan D.; McCallum, John A. Phytochemistry, 2005 , vol. 66, # 5 p. 515 - 522

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Citation Number: 46

Abstract

γ-Glutamyl transpeptidase (EC 2.3. 2.2; GGT) catalyses hydrolysis of γ-glutamyl linkages in γ- glutamyl peptides and transfer of the γ-glutamyl group to amino acids and peptides. Although plant γ-glutamyl peptide metabolism is important in biosynthesis and metabolism of secondary products and xenobiotics, plant GGTs are poorly characterised. We purified a membrane-associated GGT from sprouting onion bulbs that catalyses transpeptidation of ...