Protein-Coupled Fluorescent Probe To Visualize Potassium Ion Transition on Cellular Membranes

…, T Ueno, Y Imaizumi, T Nagano, Y Urano

Index: Komatsu, Toru; Johnsson, Kai; Okuno, Hiroyuki; Bito, Haruhiko; Inoue, Takanari; Nagano, Tetsuo; Urano, Yasuteru Journal of the American Chemical Society, 2011 , vol. 133, # 17 p. 6745 - 6751

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Citation Number: 4

Abstract

K+ is the most abundant metal ion in cells, and changes of [K+] around cell membranes play important roles in physiological events. However, there is no practical method to selectively visualize [K+] at the surface of cells. To address this issue, we have developed a protein- coupled fluorescent probe for K+, TLSHalo. TLSHalo is responsive to [K+] in the physiological range, with good selectivity over Na+ and retains its K+-sensing properties ...