A scalable, extrusion-free method for efficient liposomal encapsulation of plasmid DNA

LB Jeffs, LR Palmer, EG Ambegia, C Giesbrecht…

Index: Pharmaceutical Research, , vol. 22, # 3 p. 362 - 372

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Citation Number: 155

Abstract

... Pharmaceutical Research, Vol. ... of 55:20: 15:10 in absolute, anhydrous ethanol and then adding distilled water to achieve an ethanol concentration of 90 vol%. ... For in vivo transfection studies, SPLP (200 μl total volume containing 2 mg total lipid and 100 μg total DNA) were ad ...