Highly sensitive detection of GG mismatched DNA by surfaces immobilized naphthyridine dimer through poly (ethylene oxide) linkers

K Nakatani, A Kobori, H Kumasawa, I Saito

Index: Nakatani, Kazuhiko; Kobori, Akio; Kumasawa, Hiroyuki; Saito, Isao Bioorganic and Medicinal Chemistry Letters, 2004 , vol. 14, # 5 p. 1105 - 1108

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Citation Number: 19

Abstract

Naphthyridine dimer is a unique molecule that strongly, and selectively, binds to the guanine– guanine mismatch in duplex DNA. We have synthesized naphthyridine dimers possessing a different length of poly (ethylene oxide)(PEO) linker, and immobilized them to CM5 sensor chip to carry out a surface plasmon resonance (SPR) assay of DNA duplexes containing a single base mismatch. The sensitivity of the sensor remarkably increased with increasing ...

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