Effect of phytate on the hydrolysis of p-nitrophenyl phosphate with phosphatase from various sources

T Hayakawa, F Okada, M Tsutsui, N Sato…

Index: Hayakawa, Toshiro; Okada, Fusako; Tsutsui, Michiko; Sato, Naomi; Igaue, Ikuo Agricultural and Biological Chemistry, 1991 , vol. 55, # 3 p. 651 - 657

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Citation Number: 9

Abstract

Phosphatase activity, using p-nitrophenyl phosphate (NPP) as the substrate, was inhibited by myo-inositol hexaphosphate (phytate). The extent of inhibition by 5mm phytate of the alkaline phosphatase activity of calf intestine and Escherichia coli, and of the acid phosphatase activity of wheat germ was 82, 56 and 50%, respectively. However, acid phosphatases from rice bran were not inhibited by 10mm phytate. The effect of ...

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