Causes of unreproducibility of C. rugosa lipase-catalyzed reactions in slightly hydrated organic media

PD de María, JVS Gago

Index: Dominguez De Maria, Pablo; Sinisterra Gago, Jose V. Tetrahedron, 1999 , vol. 55, # 28 p. 8555 - 8566

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Citation Number: 20

Abstract

Lipase activity, measured as hydrolysis of tributyrin is a valid assay to quantify the lipase activity of a lyophilized crude lipase in hydrolysis reactions but it is not useful to predict the catalytic activity in lipase-catalyzed reactions in organic media. Three factors control the catalytic activity in these media: i) relative proportion of isoenzymes; ii) amount of water in the lyophilized crude enzyme and iii) amount of lipase protein in the commercial powder. ...