Tetrahedron: Asymmetry

A simple separation method for (S)-hydroxynitrile lyase from cassava and its application in asymmetric cyanohydrination

Z Zheng, Y Zi, Z Li, X Zou

Index: Zheng, Zubiao; Zi, Yan; Li, Zhongzhou; Zou, Xinzhuo Tetrahedron Asymmetry, 2013 , vol. 24, # 8 p. 434 - 439

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Citation Number: 3

Abstract

Using an acetone precipitation method, crude (S)-hydroxynitrile lyase [(S)-MeHNL] was separated from Munihot esculenta (cassava) leaves, and used directly as biocatalyst to catalyze asymmetric cyanohydrination and produce cyanohydrins with enantiomeric purities (⩾ 90% ee) significantly greater than those previously reported. The use of a water/i-Pr2O system with an enzyme, NaCN, and appropriate amounts of acetic acid is crucial in ...

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