In vitro synthesis of 1, N6??etheno??2′??deoxyadenosine and 1, N2??etheno??2′??deoxyguanosine by 2, 4??dinitrophenol and 1, 3??dinitropyrene in presence of a …

S Chiron, S Barbati, M De Meo…

Index: Chiron, Serge; Barbati, Stephane; De Meo, Michel; Botta, Alain Environmental Toxicology, 2007 , vol. 22, # 2 p. 222 - 227

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Citation Number: 24

Abstract

Abstract The formation of covalent nitro-PAH DNA adducts and nitro-PAH mediated oxidative lesions are two possible mechanisms for the initiation of nitro-PAH carcinogenesis. Sixty-minute incubation of 1, 3-dinitropyrene (100 μM) or 1, 4-dinitrophenol (100 μM) with a mixture of 150 μM NADH, 0.5 units of E. coli nitroreductase, 100 μM linoleic acid, 0.5 mM ferrous iron, and 100 μM 2′-deoxyadenosine (2′-dA) or 100 μM 2′-deoxyguanosine (2 ...

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