Liquid chromatography–mass spectrometry (LC–MS) of steroid hormone metabolites and its applications

TM Penning, SH Lee, Y Jin, A Gutierrez…

Index: Penning, Trevor M.; Lee, Seon-Hwa; Jin, Yi; Gutierrez, Alejandro; Blair, Ian A. Journal of Steroid Biochemistry and Molecular Biology, 2010 , vol. 121, # 3-5 p. 546 - 555

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Citation Number: 53

Abstract

Advances in liquid chromatography–mass spectrometry (LC–MS) can be used to measure steroid hormone metabolites in vitro and in vivo. We find that LC–electrospray ionization (ESI)-MS using a LCQ ion trap mass spectrometer in the negative ion mode can be used to monitor the product profile that results from 5α-dihydrotestosterone (DHT)-17β-glucuronide, DHT-17β-sulfate, and tibolone-17β-sulfate reduction catalyzed by human members of the ...