Journal of the American Chemical Society

Monitoring catalytic activity by immunoassay: implications for screening

G MacBeath, D Hilvert

Index: MacBeath; Hilvert Journal of the American Chemical Society, 1994 , vol. 116, # 14 p. 6101 - 6106

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Citation Number: 62

Abstract

Abstract: We have developed an immunoassay for screening large libraries of potential catalysts of a bimolecular Diels-Alder reaction. The procedure involves the reaction of immobilized dienophile with diene in solution and subsequent detection of the product with a monoclonal antibody as in a regular enzyme-linked immunosorbant assay (ELISA). The assay exhibits the expected dependence on time and diene concentration. A catalytic ...

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