Characterization of a novel Salmonella Typhimurium chitinase which hydrolyzes chitin, chitooligosaccharides and an N-acetyllactosamine conjugate

T Larsen, BO Petersen, BG Storgaard, JØ Duus…

Index: Glycobiology, , vol. 21, # 4 p. 426 - 436

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Citation Number: 14

Abstract

... Glycobiology. ... After induction, growth was continued for 21 h at 30°C. The cells were harvested by centrifugation and resuspended in 50 mL of loading buffer, 20 mM MOPS, pH 7.5, containing 0.5 M NaCl ... The reaction volume was 10 µL in 50 mM sodium phosphate buffer, pH 6.0 ...

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