Organic & biomolecular chemistry

Substituted 2-nitrobenzyltrichloroacetate esters for photodirected oligonucleotide detritylation in solid films

PJ Serafinowski, PB Garland

Index: Serafinowski, Pawel J.; Garland, Peter B. Organic and Biomolecular Chemistry, 2008 , vol. 6, # 18 p. 3284 - 3291

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Citation Number: 6

Abstract

Oligonucleotide microarray fabrication by chemical synthesis using photoacid generators in solid films could have advantages over existing methods, but has not matched the accuracy of conventional synthesis where detritylation is performed with acid solutions. To address this problem, we explored the kinetics and equilibria of nucleoside detritylation in solid films, using trichloroacetic acid (TCA) generated by photolysis from its esters with substituted 2- ...