“Click chemistry” in the preparation of porous polymer-based particulate stationary phases for μ-HPLC separation of peptides and proteins

M Slater, M Snauko, F Svec, JMJ Fréchet

Index: Slater, Michael; Snauko, Marian; Svec, Frantisek; Frechet, Jean M. J. Analytical Chemistry, 2006 , vol. 78, # 14 p. 4969 - 4975

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Citation Number: 133

Abstract

With the use of the copper (I)-catalyzed (3+ 2) azide-alkyne cycloaddition, an element of “click chemistry,” stationary phases carrying long alkyl chains or soybean trypsin inhibitor have been prepared for use in HPLC separations in the reversed-phase and affinity modes, respectively. The ligands were attached via a triazole ring to size monodisperse porous beads containing either alkyne or azide pendant functionalities. Alkyne-containing beads ...