Purification and characterization of Ocimum basilicum L. polyphenol oxidase

S Dogan, P Turan, M Dogan, O Arslan…

Index: Dogan, Serap; Turan, Pinar; Dogan, Mehmet; Arslan, Oktay; Alkan, Mahir Journal of Agricultural and Food Chemistry, 2005 , vol. 53, # 26 p. 10224 - 10230

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Citation Number: 26

Abstract

A partial characterization of polyphenol oxidase (PPO) activity in Ocimum basilicum L. is described. PPO in O. basilicum L. was extracted and purified through (NH4) 2SO4 precipitation, dialysis, and a Sepharose 4B-l-tyrosine-p-aminobenzoic acid affinity column. The samples obtained from (NH4) 2SO4 precipitation and dialysis were used for the characterization of PPO. At the end of purification by affinity chromatography, 11.5-fold ...