An improved procedure for the methylation analysis of oligosaccharides and polysaccharides

PJ Harris, RJ Henry, AB Blakeney, BA Stone

Index: Harris; Henry; Blakeney; Stone Carbohydrate research, 1984 , vol. 127, # 1 p. 59 - 73

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Citation Number: 517

Abstract

Abstract An improved procedure for the methylation analysis of oligosaccharides and polysaccharides is described. Steps in the procedure were examined and optimised for quantitative recovery and speed. Methylation was shown to be complete by using [14 C] methyl iodide. All operations were performed in the same tube and the need to concentrate solutions containing acetylated alditols of methylated sugars was eliminated, thus ...