Synthesis of a new water soluble 2, 2-bifunctionalized spin label and its application to troponin C

…, M Taki, M Nakamura, T Arata, Y Yamamoto

Index: Hirayama, Tasuku; Taki, Masayasu; Nakamura, Motoyoshi; Arata, Toshiaki; Yamamoto, Yukio Chemistry Letters, 2006 , vol. 35, # 8 p. 834 - 835

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Citation Number: 4

Abstract

A new water soluble 2, 2-bifunctionalized spin label (2, 2E-BSL) having pyrrolidine nitroxide moiety was synthesized starting from a nitro compound with two hydroxymethyl group converted to the linkers of the 2, 2E-BSL, and was applied to label troponin C (TnC). Labeled TnC through two linkages were successfully isolated, and 2, 2E-BSL was proved to be immobilized on TnC by EPR measurement.