Enzymatic synthesis of dTDP-4-amino-4, 6-dideoxy-D-glucose using GerB (dTDP-4-keto-6-deoxy-D-glucose aminotransferase)

YS Chung, DH Kim, WM Seo, HC Lee, K Liou…

Index: Chung, Young Soo; Kim, Dae Hee; Seo, Won Min; Lee, Hei Chan; Liou, Kwangkyoung; Oh, Tae-Jin; Sohng, Jae Kyung Carbohydrate Research, 2007 ,  vol. 342,  # 11  p. 1412 - 1418

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Citation Number: 11

Abstract

Over-expressed GerB (dTDP-4-keto-6-deoxy-d-glucose aminotransferase) of Streptomyces sp. GERI-155 was used in the enzymatic synthesis of dTDP-4-amino-4, 6-dideoxy-d-glucose (2) from dTDP-4-keto-6-deoxy-d-glucose (1). Five enzymes including dTMP kinase (TMK), acetate kinase (ACK), dTDP-glucose synthase (TGS), dTDP-glucose 4, 6-dehydratase (DH), and dTDP-4-keto-6-deoxy-d-glucose aminotransferase (GerB) were used to synthesize 2 ...