Protease-catalysed synthesis of peptides containing histidine and lysine

K Beck-Piotraschke, HD Jakubke

Index: Beck-Piotraschke, Karin; Jakubke, Hans-Dieter Tetrahedron Asymmetry, 1998 , vol. 9, # 9 p. 1505 - 1518

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Citation Number: 9

Abstract

The kinetically controlled α-chymotrypsin-and trypsin-catalysed syntheses of peptides starting from simple acyl donor esters containing histidine at the P1-position (nomenclature according to Schechter and Berger) and lysine derivatives as amino components were examined on the basis of their kinetic parameters. Despite higher specificity constants (kcat/KM) of trypsin-catalysed ester hydrolysis, α-chymotrypsin-catalysed acyl transfer to ...