2-(4-Tolylsulfonyl) ethoxymethyl (TEM)—a new 2′-OH protecting group for solid-supported RNA synthesis

…, D Honcharenko, J Chattopadhyaya

Index: Zhou, Chuanzheng; Honcharenko, Dmytro; Chattopadhyaya, Jyoti Organic and Biomolecular Chemistry, 2007 , vol. 5, # 2 p. 333 - 343

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Citation Number: 45

Abstract

The 2-(4-tolylsulfonyl) ethoxymethyl (TEM) as a new 2′-OH protecting group is reported for solid-supported RNA synthesis using phosphoramidite chemistry. The usefulness of the 2′- O-TEM group is exemplified by the synthesis of 12 different oligo-RNAs of various sizes (14– 38 nucleotides long). The stepwise coupling yield varied from 97–99% with an optimized coupling time of 120 s. The synthesis of all four pure phosphoramidite building blocks is ...