Xenobiotica

Identification of human cytochrome P450 isoforms involved in the metabolism of brotizolam

…, W Kishimoto, K Sakai, A Nagakura, T IGARASHI*

Index: Senda; Kishimoto; Sakai; Nagakura; Igarashi Xenobiotica, 1997 , vol. 27, # 9 p. 913 - 922

Full Text: HTML

Citation Number: 21

Abstract

1. To identify the cytochrome P450 (CYP) isoenzyme (s) responsible for the major metabolic pathways of brotizolam in man, we examined the metabolism of brotizolam using human liver microsomes and microsomes expressing individual human CYP isoenzymes (CYP1A1, 1A2, 2A6, 2B6, 2C9, 2C19, 2D6, 2E1, 3A4). 2. Brotizolam was metabolized to α-OH-and 6- OH-brotizolam by human liver microsomes (n= 3). V max for α-and 6-hydroxylation of ...