Direct selection for catalysis from combinatorial antibody libraries using a boronic acid probe: primary amide bond hydrolysis

…, BJ Lavey, CHL Lo, A Datta, P Wentworth…

Index: Gao, Changshou; Lavey, Brian J.; Lo, Chih-Hung L.; Datta, Anita; Wentworth Jr., Paul; Janda, Kim D. Journal of the American Chemical Society, 1998 , vol. 120, # 10 p. 2211 - 2217

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Citation Number: 79

Abstract

This report describes a joint hybridoma and combinatorial antibody library approach to elicit catalysts for primary amide bond hydrolysis. By immunization with a trigonal boronic acid hapten 3a and construction of a Fab (antigen-binding fragment) library, a diastereoselective catalyst for hydrolysis of the tripeptide primary amide substrate 1a was selected. In contrast, no antibody catalyst was isolated by standard hybridoma methods of monoclonal antibody ...