Chemistry & biology

Enzymatic extender unit generation for in vitro polyketide synthase reactions: structural and func-tional showcasing of Streptomyces coelicolor MatB

AJ Hughes, A Keatinge-Clay

Index: Hughes, Amanda J.; Keatinge-Clay, Adrian Chemistry and Biology, 2011 , vol. 18, # 2 p. 165 - 176

Full Text: HTML

Citation Number: 62

Abstract

In vitro experiments with modular polyketide synthases (PKSs) are often limited by the availability of polyketide extender units. To determine the polyketide extender units that can be biocatalytically accessed via promiscuous malonyl-CoA ligases, structural and functional studies were conducted on Streptomyces coelicolor MatB. We demonstrate that this adenylate-forming enzyme is capable of producing most CoA-linked polyketide extender ...

Related Articles:

Kinetics and mechanism of the thiolytic removal of the dithiasuccinoyl (Dts) amino protecting group

[Barany, George; Merrifield, R. B. Journal of the American Chemical Society, 1980 , vol. 102, # 9 p. 3084 - 3095]

Étude de germathiazolidines et de dithioacétals germaniés dérivés de la cystéamine et méthylcystéamine N-substituées: synthèse et activité radioprotectrice

[Fatome, Marc; Sentenac-Roumanou, Henri; Lion, Claude; Satge, Jacques; Rima, Ghassoub European Journal of Medicinal Chemistry, 1988 , vol. 23, p. 257 - 266]

Activation of carboxyl groups by diphenyl 2-oxo-3-oxazolinylphosphonate: Facile preparation of versatile reagents, 3-acyl-2-oxazolones

[Kunieda, Takehisa; Higuchi, Tsunehiko; Abe, Yoshihiro; Hirobe, Masaaki Tetrahedron, 1983 , vol. 39, # 20 p. 3253 - 3260]

Activation of carboxyl groups by diphenyl 2-oxo-3-oxazolinylphosphonate: Facile preparation of versatile reagents, 3-acyl-2-oxazolones

[Kunieda, Takehisa; Higuchi, Tsunehiko; Abe, Yoshihiro; Hirobe, Masaaki Tetrahedron, 1983 , vol. 39, # 20 p. 3253 - 3260]

Nonlinear structure-reactivity correlations. Acyl transfer between sulfur and oxygen nucleophiles

[Journal of the American Chemical Society, , vol. 99, p. 451 - 464]

More Articles...